970 Taxonomical clarification and overview of six cutibacterium species including recently described species cutibacterium modestum and cutibacterium porci by genomic and proteomic methods

Background: Recent descriptions of two new species genus Cutibacterium resulted in a further expansion the taxonomical tree genus, which include acnes. One species, modestum, was coined 2020 by presenter as fifth Cutibacterium, had been tentatively named “Propionibacterium humerusii”. Another porci, firstly isolated from porcine faeces Wylensek et al. 2020, being first possibly only non-human member genus. Following these findings, researchers need map status quo Methods: To describe comprehensive view and subspecies to verify their positions, we applied three several tools, are silico DNA-DNA hybridization, average nucleotide identity, MALDI-TOF mass spectrometry. Results: Our genome-based approaches (in hybridization identity) enabled subspecies, verified individual species/subspecies pairwise similarity values (species cutoff: 70% 95% identity; 79% 99%, respectively). Furthermore, proteomic approach (MALDI-TOF spectrometry) showed effective differentiation using 3,000-15,000 m/z range spectral patterns this latest identifying tool. Discussion: Dermatological see names coming years. presenting information will help them understand background get more familiarized names.